Imaging techniques to Study T1D

In this episode, Nathan Steenbuck PhD Candidate in the Bodenmiller Laboratory discusses imaging mass cytometry reveals early beta-cell dysfunction and changes in immune signatures during type 1 diabetes progression in human pancreata.

In this episode, Madeline McLaughlin joins us to discuss ultra-high resolution imaging enables quantification of beta cell ER.

#type1diabetes #type1diabetesresearch #imaging #highresolutionimaging #ultrahighresolution #quantification #betacell #betacellER #ER #endoplasmicreticulum #t1d

In this episode, Abdelfattah El Quaamari discusses Sensory-Islet Crosstalk in Glucose Homeostasis.

#asktheexpert #ATE #type1diabetes #t1d #t1dresearch #diabetesresearch #novel #diabetes #sensory #islet #isletcrosstalk #crosstalk #glucosehomeostasis #glucose #glucosecontrol #pancreasimaging #pancreas

In this episode, Matthew Merrins from University of Wisconsin-Madison and Richard Benninger from University of Colorado discuss 3D light-sheet imaging of the islet: New insights into the beta cell subpopulations that lead and coordinate Ca2+ oscillations.

#asktheexpert #ATE #type1diabetes #t1d #t1dresearch #diabetesresearch #novel #diabetes #betacells #3dlightimaging #pancreas #pancreaticimaging #calcium #calciumoscillations #betacellsubpopulations

In this episode, Andreas Müller joins us to discuss a great listener: The primary cilium as an antenna for islet intrinsic and extrinsic signals.


#asktheexpert #ATE #type1diabetes #t1d #t1dresearch #diabetesresearch #novel #diabetes #cilia #primarycilium #islet #intrinsicsignals #extrinsicsignals #signaling

One hour panel discussion of challenges, progress, and roadmap to full clarification of human pancreatic function in context of type 1 diabetes

PANELISTS include the following scientists:

Mark O Huising PhD, UC Davis CHAIR
Vira Kravets PhD, UCSD
Julia Panzer PhD, City of Hope
Adrian Villalba Felipe PhD, INSERM Paris
Jack Virostko PhD, Dell Medical School, UTexas Austin
Stan Jankauskas PhD, Einstein NY
Nicholas Mamrak, PhD JDRF/Beyond Type 1 NY

Discussion Sections
1) Challenges: 10 minutes
2) Progress: 30 minutes (sharing of panel video clips)
3) Roadmap forward: 10 minutes
4) Audience Q/A: 10 minutes

In this episode, Xavier Gidrol & Emily Tubbs discuss promises and challenges in pancreatic organoid development.


#novel #asktheexpert #ATE #T1D #diabetes #type1diabetes #human #science #novel #pancreas #pancreaticorganoid #organoid #organoiddevlopment #stemcell #stemcellresearch

In this episode, Adrián Villalba Felipe discusses human pancreas development through 3D glasses.

#novel #asktheexpert #ATE #T1D #diabetes #type1diabetes #human #science #bioimaging #pancreas #pancreaticimaging #3D #humanpancreas

In this episode, Jason Bini discusses quantitative PET imaging in humans of Pancreatic Beta Cell Mass and Function in vivo.

#novel #asktheexpert #ATE #T1D #diabetes #type1diabetes #human #science #PET #quantitativePET #petimaging #betacells #pancreas #pancreaticbetacells #invivo #invivoresearch

In this episode, Juan Dominguez, Fahd Qadir, Mayur Doke, & Isabella Altilio, a PhD student in the lab, join us to discuss pancreatic regeneration at work: lessons from dynamic scRNA sequencing of live human pancreas slices.

#cellmetabolism #scRNA #sequencing #RNAseq #singlecell #singlecellRNA #pancreas #pancreaticregeneration #regeneration #livehumanpancreas #humanpancreas #livehumanpancreasslices #pancreasslices #autoimmunity #novel #asktheexpert #ATE #T1D #type1diabetes #science

In this episode, Drs. Jing Hughes and Ben Giepmans join us to discuss advances in islet SEM: scanning below the membrane.

#SEM #islet #membrane #electronmicroscopy #cilia #advancingscience #type1diabetes #t1d #novel #science #asktheexpert #asktheexperts

Dr. Ed Phelps from University of Florida joins us to discuss pancreas slices to study interactions between immune cells and islets.

Dr. Jack Virostko joins us to discuss imaging the pancreas in T1D.

Ask the Expert is a ~30 minute digital cafe experience where scientists and grad students can meet and exchange with thought leaders in the field of type 1 diabetes. Link below to sign up for a seat in the cafe!

https://thesugarscience.org/ask-the-expert/

Dr. Mark Huising joins us to discuss observing islet cell behavior in real time.

Ask the Expert is a ~30 minute digital cafe experience where scientists and grad students can meet and exchange with thought leaders in the field of type 1 diabetes. Link below to sign up for a seat in the cafe!

https://thesugarscience.org/ask-the-expert/

Dr. Austin Lefebvre joins us to discuss mitochondrial causes and consequences in diabetes.

Ask the Expert is a ~30 minute digital cafe experience where scientists and grad students can meet and exchange with thought leaders in the field of type 1 diabetes. Link below to sign up for a seat in the cafe!

https://thesugarscience.org/ask-the-expert/

Dr. Victoria Salem joins us to discuss in-vivo evidence for islet "hub" cells.

Ask the Expert is a ~30 minute digital cafe experience where scientists and grad students can meet and exchange with thought leaders in the field of type 1 diabetes. Link below to sign up for a seat in the cafe!

https://thesugarscience.org/ask-the-expert/

Listen to a sneak peek of podcast episode 50 with Ed Boyden at MIT. Hear what he has to say about how spatial multiplexing of fluorescent reporters for imaging signaling network dynamics can help researchers studying type 1 diabetes.

Follow the link below for full podcast episode:
https://www.podbean.com/eu/pb-igyzb-f7fc44

In this video, Jacob Hecksher-Sørensen joins us to discuss imaging diabetes using light sheet microscopy. Jacob walks us through some of the advantages of using this technology and provides us with a live demonstration from two previous experiments.

Click on the link below to listen to our podcast with him.
https://thesugarscience.podbean.com/e/episode-38-jacob-hecksher-s%C3%B8rensen-principal-scientist-at-gubra-imaging-diabetes-using-light-sheet-microscopy/

In this video vLUME founders, Alex Kitching, Alex Spark, and Steven Lee join us to discuss vLUME technology, 3D Nanoimaging Platform. vLUME provides a live demo of the technology and an inside look at a helper T cell. vLUME is the world's first and only Virtual Reality solution for Super-resolution Microscopy. Visualize, segment, and analyze in real-time your massive localization data at any scale.

Click on the link below to listen to our podcast with them.
https://www.podbean.com/ew/pb-du7mt-f3bf41

Their website: https://www.lumevr.com/